The Effects of Chemical Etching and Ultra-Fine Grain Structure of Titanium on MG-63 Cells Response

In this work, we study the influence of surface properties ultrafine grained (UFG) and coarse (CG) titanium on morphology, viability, proliferation differentiation osteoblast-like MG-63 cells. Wet chemical etching in H2SO4/H2O2 NH4OH/H2O2 solutions was used for producing surfaces with varying topography, composition wettability. The topography morphology have been studied by scanning electron microscopy (SEM) atomic force (AFM). determined time flight mass-spectrometry (TOF-SIMS) X-ray photoelectron spectroscopy (XPS). results showed that it is possible to obtain samples different compositions, hydrophilicity, nanoscale or/and microscale structures changing type solution. It found developed can improve spreading rate A significant advantage UFG series comparison CG adhesion, at later stages cultivation (7 days), higher alkaline phosphatase (ALP) activity faster achievement its maximum values found. However, there no clear benefit osteopontin (OPN) expression. All cytotoxicity towards cells promoted their osteogenic differentiation.